ELISA kits | Mabtech

Why Mabtech ELISA

Our customers have chosen our ELISpot kits to detect secreted proteins for decades. And we apply the exact same scrutiny when we choose the optimal antibody pair for our ELISA kits. Often, we have validated the same antibody pair for ELISpot, FluoroSpot, and ELISA in parallel. We offer ELISAs for selected cytokines, chemokines, immunoglobulins, and apolipoproteins for a range of species.

We love to see you succeed in your ELISA experiment. Our step-by-step guide will assist you in getting it right. Additionally, our ELISA troubleshooting guide will help you to avoid and resolve errors. Ultimately, we are always here to help you.

Detect native proteins

We put considerable effort into characterizing our antibodies and finding a perfectly matched antibody pair. The use of monoclonal antibodies makes our ELISAs specific, reproducible, and sensitive. We don’t cut corners during assay development and always validate that our ELISAs recognize the native protein, i.e., those actually secreted from cells. This makes our ELISAs ready for the reality researchers are uncovering.

Native Mouse IL-22 detected with our ELISA

Prevent false-positive results

Our ELISAs are sandwich ELISAs, and therefore, the risk for interference by heterophilic antibodies must be considered. These antibodies might crosslink the assay antibodies and lead to false-positive results. For this reason, we developed a sample dilution buffer, called ELISA diluent, that reduces heterophilic antibody interference. This buffer is included in our ELISA Pro kits but can also be purchased separately.

Samples from patients containing rheumatoid factor are known to have an increased risk of generating false-positive results in ELISA. For such clinical samples, we developed specialized kits to withstand heterophilic interference even more efficiently. We call this kit format ELISA PathRF.

Comparison of a sandwich ELISA with a false-positive result due to crosslinking

"Our research group uses Mabtech products for a variety of applications, especially investigating immunological responses in wildlife. We have been able to develop new assays for detecting diseases."

Michele Miller
Faculty of Medicine and Health Sciences
Stellenbosch University, South Africa

Which ELISA kit to choose?

ELISA Flex

Flex kits are our most adaptable kit format, allowing you to customize them to your liking. Add your preferred substrate, plates, and buffers to complete this setup.

ELISA Pro

Use our ELISA Pro kits to minimize variability between different experiments. These kits are thoroughly validated and include every reagent necessary.

ELISA PathRF

We recommend using our ELISA PathRF kits when analyzing clinical samples, especially when working with specimens from patients with autoimmune diseases, as these kits are optimized to resist interference by rheumatoid factor (RF) and heterophilic antibodies.

What's included?	ELISA Flex	ELISA Pro	ELISA PathRF
ELISA plate	No	Yes: Pre-coated strip plate	Yes: Pre-coated strip plate
Capture antibody	Yes	Yes: Coated on the plate	Yes: Coated on the plate
Detection antibody	Yes	Yes	Yes: recombinant
Conjugate	Yes*	Yes	Yes
ELISA standard	Yes	Yes	Yes
Diluent to block heterophilic interference	No	ELISA diluent/ Assay diluent/Apo buffer	RF-block diluent
Substrate and buffers	No	Yes	Yes
Performance data	No	Yes	Yes
Size	Reagents for 6 and 20 plates	1,2, and 10 plates	1 plate

*Conjugate: Ig kits contain antibody-enzyme conjugates

Common question: HRP or ALP?

Our ELISA Flex kits are almost always available in two versions: they rely on either ALP or HRP for the colorimetric reaction. Looking for help deciding between the two? Check out these tips.