Welcome EYRAplex to the immunoassay family
We're introducing a brand new magnetic bead-based multiplex assay, EYRAplex. Combining Mabtech's trusted monoclonal antibody pairs with magnetic beads, EYRAplex assays are designed to deliver reliable results every time. From focused 6-plex panels to broad 30-plex formats, EYRAplex lets you analyze more with less saving you time, resources, and sample volume.
The same, highly specific mAb pairs used in our ELISpot, FluoroSpot, and ELISA assays are now available in a new multiplex format with EYRAplex. And with our recombinant mAbs and novel assay buffer, heterophilic antibody interference is a thing of the past. Keep reading to learn more!
EYRAplex kits can be used with either Mabtech EYRA™ or most flow cytometers.
How it works:
Magnetic bead-based multiplex assays have been trusted for years to help researchers detect multiple analytes in solution saving time and resources. We've simply made them better. The approach immobilizes capture antibodies onto magnetic beads with a dye ratio specific to each analyte. These capture beads are pooled together as a panel and added to a 96-well plate. Samples and standards are added where analytes are captured during incubation. The excess sample is washed away and biotinylated recombinant detection antibodies are added. The final addition of a streptavidin-PE conjugate allows for measuring the amount of captured analyte. Mean fluorescent intensity (MFI) values from data acquisition are converted into analyte concentrations based on standard curves.
EYRAplex assays have been tested and validated for use with plasma (heparin or EDTA), serum, and cell supernatants.
Bead IDs: the key to multiplexing
At the core of magnetic bead-based multiplexing lies the Bead ID. In EYRAplex assays, each magnetic bead is dyed with a unique ratio of fluorescent dyes, creating a distinct Bead ID. A specific capture antibody is immobilized on each bead type, linking the Bead ID directly to a single analyte. This elegant design enables true multiplexing allowing all analytes to be measured simultaneously in a single well using a shared PE detection signal. Mabtech EYRA™ with the RAWsphere algorithm identifies the Bead ID and quantifies the PE signal intensity, easily matching each fluorescent event to its correct analyte.
A broad range of human inflammatory panels
Each EYRAplex kit comes with everything you need to get started:
- Capture bead mix
- Detection mAb mix, biotin
- Streptavidin-PE
- Standard mixes
- Assay diluents and wash buffers
- EYRAplex plate with lid and adhesive plate covers
EYRAplex kits are also compatible with most flow cytometers for analysis.
Designed to deliver answers
Each EYRAplex panel has been meticulously designed based on in-depth analysis of the most relevant and widely studied analytes in immune responses and inflammation. Whether you’re advancing vaccines, developing therapeutics, or driving immunology research forward, these panels are built to deliver meaningful, actionable data.
The panels below are just the beginning! We're actively developing new panels for additional species and research areas. We're also open for collaborating to expedite panel availability - reach out if you're interested in partnering with us!
Experience true sensitivity
Our goal is always to design tools that simply work and give you data you can rely on. When benchmarking EYRAplex kits against other bead-based assay providers, our mAb pairs showed comparable or even superior sensitivity. Choosing the optimal mAb pairs is truly an art form we've been perfecting for over 36 years!
What does this mean for you? No guesswork, just clearer and more accurate insights into the immune mechanisms at play in your samples driving your therapies, vaccines, and research forward.
Goodbye heterophilic antibody interference
When we started on our assay development journey, we looked to current multiplex assay offerings and the issues they were facing. We quickly discovered that many assays had major issues with heterophilic antibody interference. Heterophilic antibodies, including human anti-mouse antibodies (HAMA), commonly interfere with assays, leading to false positives by cross-linking capture and detection antibodies or false negatives by blocking one of them. This issue increases in autoimmune diseases and infections, i.e., clinical samples. EYRAplex assays address this by using recombinant detection mAbs and a specialized assay buffer to block interference giving you the most accurate data possible.
Results from benchmarking tests against Competitor 1 by spiking blank samples with rheumatoid factor (RF). The RF spike significantly increased the false positive signal in Competitor 1's magnetic bead-based multiplex assay compared to EYRAplex.
Are you ready?
We’re thrilled you’ve made it this far. EYRAplex has been a labor of meticulous love, and we’re excited to finally share it with you. EYRAplex panels are preloaded in Mabtech EYRA for the smoothest and fastest magnetic bead-based multiplex assay experience available giving you more time to drive your research forward.
We also know flexibility matters. That’s why every EYRAplex assay is fully compatible with most flow cytometers. Same trusted assay, simple implementation. Flow cytometer requirements are detailed in each assay datasheet.
Ready to explore Mabtech EYRA™ or find the right EYRAplex kit for your research? Click below!
Mabtech EYRA™: a flawless flow-free multiplexing instrument
Mabtech EYRA™ revolutionizes multiplex analysis while maintaining all the benefits of magnetic bead-based assays. By using confocal microscopy to image the beads of an EYRAplex assay, we simplify multiplex analysis like never before.
Simply insert your plate and return 15 minutes later to instant results. With Mabtech EYRA™ you're free to run samples, not maintenance. No need to worry about clogs or other fluidics-related issues.
