Tell the story of every cell
FluoroSpot is the multiplex version of ELISpot. Just like in the ELISpot assay, target proteins secreted by cells are captured by specific antibodies immediately after secretion and throughout the stimulation process, making it an extremely sensitive sandwich assay. The difference to ELISpot lies in the mode of detection: where ELISpot relies on an enzymatic reaction, FluoroSpot utilizes fluorescence. This allows multiplex analyses of several analytes simultaneously, enabling studies of cell populations with different functional profiles.
Like ELISpot, FluoroSpot is useful in e.g., vaccine research and evaluation of cancer immunotherapies, but gives a broader picture of the response by enumerating highly functional triple-secreting IFN-γ/IL-2/TNF-α cells, or killer cells secreting IFN-γ and Granzyme B simultaneously.
Why Mabtech FluoroSpot?
Mabtech's FluoroSpot kits are trusted by academics, clinical trial labs, pharmaceutical companies, and CROs alike for their robustness and superior sensitivity in detecting antigen-specific immune responses at a single-cell level. Allowing for polyfunctional analysis of T cells and multiple B cell isotypes, FluoroSpot is ideal for tracking immune responses throughout pre-clinical studies and clinical trials. Whether you're assessing vaccine efficacy or monitoring cell therapy potency, Mabtech's FluoroSpot kits seamlessly integrate into immune monitoring workflows, providing the high-quality, reliable data crucial for advancing therapeutic success.
"It was very beneficial to be able to do four analytes simultaneously using a 4-color FluoroSpot assay"
Sofie Pattyn
ImmunXperts, Belgium
"Using IRIS was my first experience with ELISpot or FluoroSpot, and our experiments worked right from the start."
Rocio Laguna
Immunodeficiencies and Transplant Immunology Research Group
Hospital Universitario 12 de Octubre, Spain
Different secretory profiles provide insights into the CTL or T helper cell response.
Polyfunctional T cell profiling
FluoroSpot's advanced multiplexing allows for the detailed analysis of secretory profiles of individual immune cells, providing key insights into immune system dynamics, such as Th1 or Th2 responses, or cytotoxic T lymphocyte activity. This detection of multiple cytokines enables a more nuanced understanding of immune cell function and the orchestration of immune responses, crucial for refining strategies in vaccine research and the development of novel immunotherapies. The assay can facilitate a deeper characterization of the immune response, leading to enhanced immune profiling.
Enhanced B cell profiling
FluoroSpot excels in B cell profiling by capturing multiple immunoglobulin classes or isotypes directly upon secretion and enables the detection of multiple antigen-specific antibody responses. The assay makes it easy to identify rare antigen-specific antibody-secreting cells (ASCs) with high sensitivity and allows for the assessment of circulating memory B cells. B cell FluoroSpot is particularly valuable for analyzing the breadth and specificity of B cell responses following infection or vaccination. Learn more about B cell profiling here.
B cell ELISpot and FluoroSpot can be used to measure both acute and memory B cell responses. Both total and antigen-specific B cells can be enumerated.
FluoroSpot kit formats
Build your own FluoroSpot kit with thousands of combinations to choose from. Choose your analytes, and the appropriate plates and stimuli are included.
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 1- or 10-plate kits
- Bulk options available upon request
Our most popular FluoroSpot kits with validated analyte combinations and everything you need for the assay including a positive control (for human and NHP kits).
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 2- or 10-plate kits
- Bulk options available upon request
A complete FluoroSpot kit to answer your specific scientific question with validated analyte combinations, an included PepPool and positive control. Just add cells!
Total assay time:
- 3-day protocol
- 2-step detection
Kit size options:
- 1-plate kit
- Bulk options available upon request
Can't find a FluoroSpot Plus combination that suits your study? We offer custom coating services for most analyte combinations. Just contact your local account manager and we can discuss the project further!
IRIS 2 is ready for automation with the implementation of robotic arm plate loading.
Mabtech IRIS 2 – a new era of ELISpot and FluoroSpot
Unlock the full potential of your ELISpot or FluoroSpot assays with Mabtech IRIS 2. Its unmatched accuracy, combined with user-friendly software, transforms complex data into clear and tangible results, making it the ELISpot and FluoroSpot reader for cutting-edge research.
Learn why IRIS 2 sets a new standard for ELISpot and FluoroSpot readers in both precision and ease of use. Connect with our team for a demo and see how IRIS 2 can transform your research or be implemented into automated setups.
At Mabtech, we aim for awesome every single time
Find antigen-specific cells
With ELISpot and FluoroSpot, you can detect any cell secreting the analyte or analytes of interest. The assays are considered the gold standard for detecting and enumerating antigen-specific T cells and B cells. With FluoroSpot, you get even more information by multiplexing to detect multiple secretory profiles or antigen-specific cells. We've made it easy to design your assay setup for success. Learn more in our Knowledge Hub or download our step-by-step FluoroSpot guide below.
User-friendly software
Building on 35+ years of ELISpot and FluoroSpot technology, we created a reader to meet our high standards. Introducing the next-generation ELISpot and FluoroSpot reader IRIS 2, powered by the innovative RAWSpot™️ technology, designed to deliver unmatched accuracy and sensitivity in spot detection. Relative spot volume provides a new data point in ELISpot and FluoroSpot giving a brand new dimension to the assays. We paired our novel detection algorithm with the most intuitive software available. Simply load your plate, hit "Read," and get your results. With IRIS 2, it's not just about reading spots – it's about pioneering a new era of ELISpot and FluoroSpot analysis.
We're here to help
At Mabtech, our mission is to aid researchers in reaching their goals. We do this not only by providing the best possible monoclonal antibodies, immunoassay kits, and instruments but also through webinars, educational blogs, workshops, and attending world-renowned immunology meetings. Check out our entire Knowledge Hub for inspiration, troubleshooting, guides, and more!
