IL-21 is an important regulating cytokine affecting both innate and adaptive immune responses. To enable detection of IL-21, Mabtech provides both ELISA and ELISpot kits. We are happy to introduce also alternative applications for our IL-21 monoclonal antibodies:
- Retain IL-21 in cell culture supernatants for improved detection in ELISA
- Neutralize IL-21 bioactivity
During cell culture, secreted IL-21 may be consumed by IL-21R-bearing cells, rendering it difficult to detect by ELISA. To overcome this and thus increase detectable IL-21 levels in cell culture supernatants, Mabtech’s biotinylated mAb MT21.3m can be included during cell culture (Huang et al., 2014). This mAb will bind IL-21 and prevent it from interacting with its receptor, effectively keeping it in solution to be quantified by ELISA (Fig. 1).
In addition, we provide a mixture of mAbs MT216G/21.3m with the ability to neutralize IL-21 bioactivity on human cells naturally expressing the IL-21 receptor (Huang et al., 2014) (Fig. 2).
Fig. 1. mAb MT21.3m-biotin binds to IL-21 and keeps it in solution to be quantified by ELISA
Fig. 2. mAbs MT216G/21.3m bind to IL-21 and neutralizes its bioactivity