ELISpot Path: EBV Human IFN-γ

ELISpot Path: EBV Human IFN‑γ

3420-2AST-P5-1

Publications: 1
Documents
Questions?
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolEBV (CD4 and CD8), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to EBV peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The EBV defined peptide pool contains 100 peptides derived from 22 of the latent and lytic EBV proteins. Of the peptides, 66 are MHC class I restricted, and 34 are MHC class II restricted.

Recommendation

Product details

ProductELISpot Path: EBV Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the EBV defined peptide pool (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research, Published October 28, 2024

EBV cross-reactivity in MS: A FluoroSpot study
This study explores the link between Epstein-Barr virus (EBV) and multiple sclerosis (MS), highlighting antibody and T cell cross-reactivity between EBNA1 and CRYAB. FluoroSpot technology confirmed specific T cell responses, suggesting EBV-related immune responses contribute to MS development.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458