PepPool: SARS‑CoV‑2 (S1), scanning

Human PBMC (300,000 cells/well) from a convalescent individual, previously confirmed CODID-19 positive with PCR, were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb and the number of cells secreting IFN-γ was analyzed by ELISpot.
Human PBMC from three COVID-19 convalescent individuals, previously confirmed SARS-CoV-2 positive with PCR, and PBMC from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

PepPool: SARS‑CoV‑2 (S1), scanning

3629-1

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against SARS-CoV-2 (S1 domain of spike protein) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The SARS-CoV-2 (S1) scanning pool induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human T cells. It contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides). The mean purity of the synthetic peptides is 80%.

Peptides included in the peptide pool are listed in the datasheet.

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Product details

ProductPepPool: SARS-CoV-2 (S1), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 166 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt.

Human PBMC (300,000 cells/well) from a convalescent individual, previously confirmed CODID-19 positive with PCR, were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb and the number of cells secreting IFN-γ was analyzed by ELISpot.
Human PBMC (300,000 cells/well) from a convalescent individual, previously confirmed CODID-19 positive with PCR, were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb and the number of cells secreting IFN-γ was analyzed by ELISpot.
Human PBMC (300,000 cells/well) from a convalescent individual, previously confirmed CODID-19 positive with PCR, were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb and the number of cells secreting IFN-γ was analyzed by ELISpot.
Human PBMC from three COVID-19 convalescent individuals, previously confirmed SARS-CoV-2 positive with PCR, and PBMC from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research

Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.
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