FluoroSpot Plus: Human IFN-γ/IL-10

FluoroSpot Plus: Human IFN‑γ/IL‑10

FSP-0107-2

Publications: 8

Documents

Questions?

Ensure reproducibility when diving into a multiplex analysis.

Components

Plate	Pre-coated FluoroSpot plate (mAbs 1-D1K and 9D7)
Detection mAbs	Anti-IFN-γ mAb (7-B6-1), BAM
	Anti-IL-10 mAb (12G8), biotin
Fluorophore conjugates	Anti-BAM mAb, 490
	SA-550
Buffer/Solution	FluoroSpot enhancer
Positive control	Anti-CD3 mAb (CD3-2)
Co-stimulus	Anti-CD28 mAb (CD28-A)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and IL-10 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Human IFN-γ/IL-10
Application	FluoroSpot
Analyte	IFN-γ, IL-10
Reactivity	Human
Specificity	This kit is based on matched pairs of mAbs specific for human IFN-γ and IL-10.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

PBMC (250,000 cells/well) were incubated in the absence or presence of PPD and the number of cells secreting IFN-γ and/or IL-10 was analyzed by FluoroSpot. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550 and Cy3 (IL-10). Spot analysis was made with Mabtech IRIS.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 24, 2022

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Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Product highlights

Highlighted research, Published April 5, 2019

FluoroSpot more sensitive than flow cytometry

FluoroSpot assay detected cytokine-secreting cells even when as few as 10 transfected cells were added to the cell suspension. In contrast, at least 5 000 transfected cells were required to detect the cytokines by flow cytometry.

Publications (8)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458

IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell type	T cell, Treg, Monocyte/MΦ, mDC
Gene ID	3586