Anti-bovine IFN-γ mAb (MT307), PF647P

3119-72-100T

Publications: 1

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Content

Monoclonal antibody MT307, conjugated to PF647P fluorophore. Ready to use.

In stock

Delivery 4-9 business days

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Complementary products

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Isotype control mAb (MT128) for mouse IgG1, PF647P

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IFN-γ-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

Product	Anti-bovine IFN-γ mAb (MT307), PF647P
Application	Flow cytometry
Analyte	IFN-γ
Antibody	MT307
Conjugate	PF647P
Clonality	Monoclonal
Immunogen	Recombinant bovine IFN-γ
Host	Mouse
Isotype	IgG2a
Reactivity	Cow, Sheep, Goat
Specificity	Native and recombinant bovine (cow) IFN-γ
Cross-reactivity	Cross-reacts with IFN-γ from sheep and goat. For more information, please check the Veterinary cross-reactivity guide.
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT307, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of IFN-γ by flow cytometry in viable bovine PBMC. Stimulated, fixed and permeabilized cells were stained with anti-bovine mAb MT307-PF647P (left panel). MT307-PF647P blocked with recombinant antigen prior to labelling of cells (middle panel). Matched isotype control antibody (right panel). Flow cytometry was performed on a BD FACSVerse system.

Documents

Datasheet/Protocol
SDS

Veterinary cross-reactivity guide

Publications (1)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	281237, 443396