Anti-bovine IgG mAb (MT391), biotin

3150-6-250

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Content

Monoclonal antibody MT391, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-bovine IgG mAb (MT134), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IgG in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT391 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT134 (product code 3150-3).

Product details

Product	Anti-bovine IgG mAb (MT391), biotin
Application	ELISpot, ELISA
Analyte	IgG
Antibody	MT391
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native bovine IgG
Host	Rat
Isotype	IgG1
Reactivity	Cow
Specificity	Bovine (cow) IgG
Cross-reactivity	No cross-reactivity (less than 0.1%) with bovine IgA or IgM. No cross-reactivity (less than 0.1%) with IgG from sheep, goat, horse, mouse, rat, or human. For more information, please check the Veterinary cross-reactivity guide.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT391, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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Tutorials

Tutorial, Published December 28, 2023

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Analyte information

IgG

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Alternative names	Immunoglobulin G, IgG
Cell type	B cell
Gene ID	281850, 281851, 281852