Anti-bovine IL-2 mAb (MT3B3), PF647P

Anti‑bovine IL‑2 mAb (MT3B3), PF647P

3111-72-100T

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Content

Monoclonal antibody MT3B3, conjugated to PF647P fluorophore. Ready to use.

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Anti-bovine IL-2 mAb (MT3B3), PF488P


Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IL-2-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

ProductAnti-bovine IL-2 mAb (MT3B3), PF647P
ApplicationFlow cytometry
AnalyteIL-2
AntibodyMT3B3
ConjugatePF647P
ClonalityMonoclonal
ImmunogenRecombinant bovine IL-2
HostMouse
IsotypeIgG1
ReactivityCow, Sheep
Specificity

Native and recombinant bovine (cow) IL-2

Supplied in

PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT3B3, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8 °C.

Shelf lifeAt least 18 months from date of receipt.
Detection of IL-2 by flow cytometry in viable bovine lymphocytes. Stimulated, fixed and permeabilized cells were stained with anti-bovine mAb MT3B3-PF647P (solid line). Matched isotype control antibody (dashed line). Flow cytometry was performed on a BD FACSVerse system.
Datasheet/Protocol
SDS
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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID280822
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Anti-bovine IL-2 mAb (MT3B3), unconjugated