Anti-canine IFN-γ mAb (MT166), biotin

Anti‑canine IFN‑γ mAb (MT166), biotin

3113-6-250

Publications: 7
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Validated for our assays.

Content

Monoclonal antibody MT166, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This monoclonal antibody enables specific detection of canine IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT166 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT13 (product code 3113-3).

Product details

ProductAnti-canine IFN-γ mAb (MT166), biotin
ApplicationELISpot, ELISA
AnalyteIFN-γ
AntibodyMT166
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant equine IFN-γ
HostMouse
IsotypeIgG2a
ReactivityDog
Specificity

Native and recombinant dog IFN-γ. Native and recombinant equine (horse) IFN-γ.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT166, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Highlighted research, Published May 28, 2019

Canine ELISA in drug discovery
Controlling fusion protein stability and pharmacokinetic with Mabtech ELISA Flex: Canine IFN-γ.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFN-G
Cell typeT cell, Tc, Th1, NK cell
Gene ID403801
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Anti-canine IFN-γ mAb (MT13), unconjugated