Anti-equine IFN-γ mAb (MT166), PF488P

Anti‑equine IFN‑γ mAb (MT166), PF488P

3117-71-100T

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Content

Monoclonal antibody MT166, conjugated to PF488P fluorophore. Ready to use.

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Delivery 4-9 business days

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Complementary products

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Isotype control mAb (97B4) for mouse IgG2a, PF488P

Product specifications

Intended use

This monoclonal antibody enables specific detection of equine IFN-γ-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.

Product details

Product	Anti-equine IFN-γ mAb (MT166), PF488P
Application	Flow cytometry
Analyte	IFN-γ
Antibody	MT166
Conjugate	PF488P
Clonality	Monoclonal
Immunogen	Recombinant equine IFN-γ
Host	Mouse
Isotype	IgG2a
Reactivity	Horse
Specificity	Native and recombinant equine (horse) IFN-γ
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT166, conjugated to PF488P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of IFN-γ by flow cytometry in viable equine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4% paraformaldehyde and saponin, and subsequently stained with MT166-PF488P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

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Datasheet/Protocol
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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	100034181