Anti-equine IFN-γ mAb (MT13), biotin

Anti‑equine IFN‑γ mAb (MT13), biotin

3117-6-250

Publications: 17
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Content

Monoclonal antibody MT13, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

Shipping $0

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Product specifications

Intended use

This monoclonal antibody enables specific detection of equine IFN-γ in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT13 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT166 (product code 3117-3).

Product details

ProductAnti-equine IFN-γ mAb (MT13), biotin
ApplicationELISpot, ELISA
AnalyteIFN-γ
AntibodyMT13
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant bovine IFN-γ
HostMouse
IsotypeIgG1
ReactivityHorse, Rhinoceros
Specificity

Native and recombinant equine (horse) IFN-γ. Native and recombinant bovine (cow) IFN-γ.

Cross-reactivity

Cross-reacts with IFN-γ from rhinoceros and dog. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT13, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID100034181
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