Anti-ferret IFN-γ mAb (MTF19), biotin

Anti-ferret IFN-γ mAb (MTF19), biotin

3112-6-250

Publications: 19
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Validated for our assays.

Content

Monoclonal antibody MTF19, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-ferret IFN-γ mAb (MTF14), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of ferret IFN-γ in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MTF19 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MTF14 (product code 3112-3).

Product details

ProductAnti-ferret IFN-γ mAb (MTF19), biotin
ApplicationELISpot, ELISA
AnalyteIFN-γ
AntibodyMTF19
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant ferret IFN-γ
HostMouse
IsotypeIgG1
ReactivityFerret
SpecificityNative and recombinant ferret IFN-γ
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MTF19, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFI, IFNgamma, TCRalpha
Cell typeT cell, Tc, Th1, NK cell
Gene ID101693741
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ELISA Flex: Ferret IFN-γ (HRP)