Anti-ferret IL-2 mAbs (MT264/271), unconjugated

Anti-ferret IL-2 mAbs (MT264/271), unconjugated

3104-3-250

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Anti-porcine IL-2 mAb (MT265), biotin


Product specifications

Intended use

These monoclonal antibodies enables specific detection of ferret IL-2 in ELISpot.

Serum/Plasma samples

Recommendation

MT264/271 are recommended as capture mAbs in ELISpot in combination with detection mAb MT265 (product code 3140-6).

Product details

ProductAnti-ferret IL-2 mAbs (MT264/271), unconjugated
ApplicationELISpot
AnalyteIL-2
AntibodyMT264/271
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant porcine IL-2
HostMouse
IsotypeIgG1
ReactivityFerret
SpecificityNative ferret IL-2. Native and recombinant porcine (pig) IL-2.
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibodies MT264/271, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/ProtocolSDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, POIL2, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID396868, 101674110