Anti-guinea pig IFN-γ mAb (MT4A27), PF647P

Anti‑guinea pig IFN‑γ mAb (MT4A27), PF647P

3124-72-100T

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Content

Monoclonal antibody MT4A27, conjugated to PF647P fluorophore. Ready to use.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of guinea pig IFN-γ-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

ProductAnti-guinea pig IFN-γ mAb (MT4A27), PF647P
ApplicationFlow cytometry
AnalyteIFN-γ
AntibodyMT4A27
ConjugatePF647P
ClonalityMonoclonal
ImmunogenRecombinant guinea pig IFN-γ
HostMouse
IsotypeIgG1
ReactivityGuinea pig
Specificity

Native and recombinant IFN-γ from guinea pig

Supplied in

PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT4A27, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8 °C.

Shelf lifeAt least 18 months from date of receipt.
Detection of IFN-γ by flow cytometry in guine pig spleen cells. Cells were stimulated for 16 h in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4% paraformaldehyde and saponin, and subsequently stained with MT166-PF647P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
Datasheet/Protocol
SDS
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID100379568
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Anti-guinea pig IFN-γ mAb (MT4A27), PF488P