Anti-human apoM mAb (M5), unconjugated

Anti‑human apoM mAb (M5), unconjugated

3716-3-250

Publications: 3

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Validated for our assays.

Content

Monoclonal antibody M5 unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoM mAb (M7), biotin

Product specifications

Intended use

This monoclonal antibody enables specific detection of human apoM in immunoassays such as ELISA, immunoprecipitation and Western blot.

Serum/Plasma samples

Recommendation

M5 is recommended as capture mAb in ELISA in combination with biotinylated detection mAb M7 (product code 3716-6). The antibody is also suitable for immunoprecipitation and Western blot.

Product details

Product	Anti-human apoM mAb (M5), unconjugated
Application	ELISA, Western blot
Analyte	apoM
Antibody	M5
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Recombinant human apoM
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human apoM
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody M5 unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of human apoM with mAb M5 in Western blot. 0.1 µl human plasma and 5ng purified apoM in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoM was detected using 2 µg/ml M5 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 14, 2022

Apolipoprotein dilution

Recommendations for Mabtech's apolipoprotein ELISAs

Publications (3)

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Analyte information

apoM

Analyte description	Apolipoprotein M (apoM) is a lipocalin mainly produced by the liver. It binds sphingosine-1-phosphate (S1P), and HDL-containing apoM is the major transporter of S1P. ApoM–S1P is involved in several signaling pathways implicated in the atheroprotective functions of HDL. ApoM has a role in lipoprotein metabolism, atherosclerosis, diabetes, and renal diseases. The normal concentration of apoM in human plasma is approximately 6-60 mg/L.
Alternative names	Apolipoprotein M, apoM, apolipoproteinM, apo M, G3a, HSPC336, NG20, apo-M
Cell type	B cell, Monocyte/MΦ, mDC
Gene ID	55937