Anti-human CD3 mAb (CD3-2), azide free

Anti‑human CD3 mAb (CD3‑2), azide free

3605-1-1000

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Content

Monoclonal antibody CD3-2, unconjugated. Supplied at 1 mg/ml in sterile filtered PBS, free of preservatives.

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Delivery 4-9 business days

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Product specifications

Intended use

This monoclonal antibody enables activation of human T cells in immunoassays such as ELISpot and FluoroSpot.

Serum/Plasma samples

Recommendation

CD3-2 can be used for cell stimulation in solution or on a solid phase. Recommended concentration for optimal cytokine production is 100 ng/ml. The antibody is also useful for immunohistochemistry. For flow cytometry, stain cells before paraformaldehyde fixation.

Product details

ProductAnti-human CD3 mAb (CD3-2), azide free
AnalyteCD3
AntibodyCD3-2
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenStimulated human leukocytes
HostMouse
IsotypeIgG2a
ReactivityHuman
Specificity

The ε chain of human CD3

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration1 mg/ml
Supplied in

PBS, free of preservatives. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody CD3-2, unconjugated. Supplied at 1 mg/ml in sterile filtered PBS, free of preservatives.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing. 

Shelf lifeAt least 12 months from date of receipt.
Datasheet/Protocol
SDS
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CD3

Analyte description

CD3 (cluster of differentiation 3) is a T cell co-receptor that plays an important role in T cell activation and signal transduction. The CD3 complex is composed of four chains: two CD3ε, one CD3γ, and one CD3δ chain. CD3 forms together with the TCR (α/β or γ/δ heterodimer) and two zeta (ζ) chains the T cell receptor (TCR) complex. CD3 belongs to the immunoglobulin superfamily. 

Alternative namesCluster of differentiation 3, CD3, CD3epsilon, IMD18, T3E, TCRE
Cell typeT cell, Tc, Th1, Th2, Th17, Treg, Tfh
Gene ID916