Anti-human IFN-α mAb (MT1), unconjugated

Anti‑human IFN‑α mAb (MT1), unconjugated

3423-3-250

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Content

Monoclonal antibody MT1, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IFN-α mAb (MT2), biotin


Product specifications

Intended use

This monoclonal antibody enables specific detection of human IFN-α (subtype 2) in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT1 is recommended as capture mAb in ELISpot and ELISA in combination with detection mAb MT2 (product code 3423-6).

Product details

ProductAnti-human IFN-α mAb (MT1), unconjugated
ApplicationELISpot, ELISA
AnalyteIFN-α2
AntibodyMT1
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant human IFN-α 2c
HostMouse
IsotypeIgG1
ReactivityHuman
SpecificityNative and recombinant human IFN-α subtypes 2a, 2b and 2c
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration1 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT1, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IFN-α2

Analyte description

Interferon-α (IFN-α) is a type I interferon. In humans, there are 13 IFN-α subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21. Like the other subtypes, interferon-α2  is mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. The binding of IFN-α2 to its receptor thus initiates an anti-viral response, but also anti-tumor effects have been shown. Being the first to be discovered, IFN-α2 is the most widely studied subtype. It has been used as a cytokine therapy versus hepatitis C virus infections.

Alternative namesInterferon-α2, Interferon-alpha2, IFN-α2, IFN-alpha2, IFN-a2, IFNA2, IFN-alpha-2, IFN-alphaA, IFNAB, leIF A
Cell typepDC
Gene ID3440