Anti-human IgA mAb (MT20), biotin

Anti‑human IgA mAb (MT20), biotin

3860-6-250

Publications: 35

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Validated for our assays.

Content

Monoclonal antibody MT20, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Complementary products

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Anti-human IgA mAb (MT57), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgA in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT20 is recommended as detection mAb in ELISpot in combination with capture mAb MT57 (product code 3860-3). The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-human IgA mAb (MT20), biotin
Application	ELISpot, ELISA
Analyte	IgA
Antibody	MT20
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgA
Host	Rat
Isotype	IgG1
Reactivity	Human
Specificity	Human IgA
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT20, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

mAb guides

mAb guide, Published November 14, 2022

Human IgA mAb guide

Learn how to combine Mabtech’s antibodies for human IgA ELISA and ELISpot. Mabtech’s monoclonal antibodies are specific for human IgA and offer detection of total IgA as well as antigen-specific IgA in ELISA and ELISpot.

Publications (35)

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Analyte information

IgA

Analyte description	IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in secretions and in the gastrointestinal and urogenital tract. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
Alternative names	Immunoglobulin A, IgA, A2M, IgA1
Cell type	B cell
Gene ID	3494, 3493