Anti-human IgA1 mAb (MT50), biotin

Anti‑human IgA1 mAb (MT50), biotin

3861-6-250

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Content

Monoclonal antibody MT50, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IgA1 mAb (MT50), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgA1 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT50 is recommended as detection mAb in ELISpot in combination with capture mAb MT57 (product code 3860-3). The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-human IgA1 mAb (MT50), biotin
ApplicationELISpot, ELISA
AnalyteIgA1
AntibodyMT50
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative human IgA
HostRat
IsotypeIgG1
ReactivityHuman
SpecificityHuman IgA1 derived from serum and colostrum
Cross-reactivityNo cross-reactivity (less than 0.1%) with human IgA2, IgG1, IgG2, IgG3, IgG4, IgE, or IgM
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT50, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgA1

Analyte description

IgA1 (Immunoglobulin A1) is one of the two existing IgA isotypes. The proportions of IgA1 and IgA2 secreting B cells differs in various body tissues. Although IgA1 is the most common IgA subclass in serum, IgA represents only 10-15% of the total serum Ig molecules. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.

Alternative namesImmunoglobulin A1, IgA1
Cell typeB cell
Gene ID3493