Anti-human IL-17A mAb (MT504), azide free

Anti-human IL-17A mAb (MT504), azide free

3520-5N-500

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Content

Monoclonal antibody MT504, unconjugated, Supplied at 0.5 mg/ml in PBS, free of preservatives.

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Product specifications

Intended use

This monoclonal antibody enables neutralization of human IL-17A bioactivity.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

ProductAnti-human IL-17A mAb (MT504), azide free
ApplicationNeutralization
AnalyteIL-17A
AntibodyMT504
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant human IL-17A
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Native and recombinant human IL-17A and IL-17A/F. The antibody binds to the A subunit of the IL-17A/F heterodimer.

Cross-reactivity

Cross-reacts with IL-17A from rhesus macaque, cynomolgus macaques, and common marmoset. Cross-reactivity has been tested in ELISA or ELISpot. For information on reactivity with other non-human primates (NHP), please check the NHP cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS, free of preservatives. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT504, unconjugated, Supplied at 0.5 mg/ml in PBS, free of preservatives.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 12 months from date of receipt.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

 Rhesus MacaqueCynomolgus MacaquePig-tailed MacaqueBaboon*Sooty MangabeyAfrican Green MonkeyNight Monkey*Common MarmosetSquirrel Monkey
ASSAYOld worldNew world
ApoA1      
ApoB       
ApoE (NHP)       
ApoE       
ApoH       
CCL2 (MCP-1)       
CCL4 (MIP-1β) ( ) ( )( )( )( )
CCL22 (MDC) ( ) ( )( )( )( )
CD25       
GM-CSF( )( )   
Granzyme A        
Granzyme B (NHP)       
Granzyme B     
IFN-α2        
IFN-α pan   ( )   
IFN-γ (NHP)
IFN-γ( )
IgA (NHP)       
IgA       
IgG       
IgM       
INS     
IL-1α        
IL-1β        
IL-2 (NHP)
IL-3       
IL-4( )( )( )( )
IL-5( )( )( ) 
IL-6( )( ) ( ) 
IL-8 (NHP)        
IL-8 (ELISA)        
IL-8 (ELISpot)        
IL-10 (NHP)      
IL-10       
IL-12/-23 (p40)      
IL-12 (p70)        
IL-13 (NHP)( )( )( )  
IL-13        
IL-17A (NHP)( )( )   
IL-17A     
IL-17F        
IL-17A/F        
IL-21      
IL-22        
IL-23     
IL-27        
IL-31        
IP-10       
Perforin( )   
TGF-β1 (latent)       
TNF-α (NHP)( )  ( )( )( )( )
TNF-α       
          
SINGLE mAbs         
Cell stimulation         
CD3, mAb CD3-1       
CD3, mAb CD3-2       
CD28, mAb CD28-A       
Flow cytometry          
IFN-γ mAb 1-D1K( )( )( )( )( )( )
IL-2 mAb MT8G10( )( )( )( ) ( )( )( )
IL-4 mAb IL4-3( )       
IL-17A mAb MT504        
Perforin mAb Pf-344     
TNF-α mAb MT15B15       
Ig reagents         
IgG1 mAb MTG1218       
IgG1 mAb MT1939       
IgG2 mAb H6200       
IgG2 mAb MTG211E       
IgG3 mAb MTG34       
IgG4 mAb MTG42       
Western blot         
IFN-γ mAb MT111W( )( )( )( )( )( )( )( )( )
TNF-α mAb MT15B15( )( )( )  ( )( )( )( )

Symbol keys:


Cross-reactivity

Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

  • good
  • poor
  • no

Indications of Cross-reactivity

Based on reports from others or by analysis using recombinant proteins.

  • ( ) good
  • ( ) poor
  • ( ) no

Monkey kits

Specifically developed monkey kits are marked with (NHP).

Blank boxes

Represent cross-reactivities not evaluated.

* Comprises several species. Cross-reactivity may have to be verified on a species basis.

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IL-17A

Analyte description

Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.

Alternative namesInterleukin 17A, IL-17A, IL17A, CTLA-8, CTLA8, IL-17, IL17, ILA17
Cell typeTh17
Gene ID3605