Anti-monkey CD3 mAb (CD3-1), azide free

Anti-monkey CD3 mAb (CD3-1), azide free

3610-1-50

Publications: 2
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Validated for our assays.

Content

Monoclonal antibody CD3-1, unconjugated. Supplied at 100 µg/ml in PBS, free of preservatives

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Delivery 4-9 business days

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Product specifications

Intended use

This monoclonal antibody enables activation of monkey T cells in immunoassays such as ELISpot and FluoroSpot.

Serum/Plasma samples

Recommendation

Recommended concentration for optimal cytokine production is 100 ng/ml.

Product details

ProductAnti-monkey CD3 mAb (CD3-1), azide free
ApplicationT cell stimulation
AnalyteCD3
AntibodyCD3-1
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenStimulated human leukocytes
HostMouse
IsotypeIgG3
ReactivityNon-human primates
Specificity

Rhesus and cynomolgus macaque CD3. Human CD3.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration100 µg/ml
Supplied in

PBS, free of preservatives. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody CD3-1, unconjugated. Supplied at 100 µg/ml in PBS, free of preservatives

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 12 months from date of receipt.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

 Rhesus MacaqueCynomolgus MacaquePig-tailed MacaqueBaboon*Sooty MangabeyAfrican Green MonkeyNight Monkey*Common MarmosetSquirrel Monkey
ASSAYOld worldNew world
ApoA1      
ApoB       
ApoE (NHP)       
ApoE       
ApoH       
CCL2 (MCP-1)       
CCL4 (MIP-1β) ( ) ( )( )( )( )
CCL22 (MDC) ( ) ( )( )( )( )
CD25       
GM-CSF( )( )   
Granzyme A        
Granzyme B (NHP)       
Granzyme B     
IFN-α2        
IFN-α pan   ( )   
IFN-γ (NHP)
IFN-γ( )
IgA (NHP)       
IgA       
IgG       
IgM       
INS     
IL-1α        
IL-1β        
IL-2 (NHP)
IL-3       
IL-4( )( )( )( )
IL-5( )( )( ) 
IL-6( )( ) ( ) 
IL-8 (NHP)        
IL-8 (ELISA)        
IL-8 (ELISpot)        
IL-10 (NHP)      
IL-10       
IL-12/-23 (p40)      
IL-12 (p70)        
IL-13 (NHP)( )( )( )  
IL-13        
IL-17A (NHP)( )( )   
IL-17A     
IL-17F        
IL-17A/F        
IL-21      
IL-22        
IL-23     
IL-27        
IL-31        
IP-10       
Perforin( )   
TGF-β1 (latent)       
TNF-α (NHP)( )  ( )( )( )( )
          
SINGLE mAbs         
Cell stimulation         
CD3, mAb CD3-1       
CD3, mAb CD3-2       
CD28, mAb CD28-A       
Flow cytometry          
IFN-γ mAb 1-D1K( )( )( )( )( )( )
IL-2 mAb MT8G10( )( )( )( ) ( )( )( )
IL-4 mAb IL4-3( )       
IL-17A mAb MT504        
Perforin mAb Pf-344     
TNF-α mAb MT15B15       
Ig reagents         
IgG1 mAb MTG1218       
IgG1 mAb MT1939       
IgG2 mAb H6200       
IgG2 mAb MTG211E       
IgG3 mAb MTG34       
IgG4 mAb MTG42       
Western blot         
IFN-γ mAb MT111W( )( )( )( )( )( )( )( )( )
TNF-α mAb MT15B15( )( )( )  ( )( )( )( )

Symbol keys:


Cross-reactivity

Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

  • good
  • poor
  • no

Indications of Cross-reactivity

Based on reports from others or by analysis using recombinant proteins.

  • ( ) good
  • ( ) poor
  • ( ) no

Monkey kits

Specifically developed monkey kits are marked with (NHP).

Blank boxes

Represent cross-reactivities not evaluated.

* Comprises several species. Cross-reactivity may have to be verified on a species basis.

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CD3

Analyte description

CD3 (cluster of differentiation 3) is a T cell co-receptor that plays an important role in T cell activation and signal transduction. The CD3 complex is composed of four chains: two CD3ε, one CD3γ, and one CD3δ chain. CD3 forms together with the TCR (α/β or γ/δ heterodimer) and two zeta (ζ) chains the T cell receptor (TCR) complex. CD3 belongs to the immunoglobulin superfamily. 

Alternative namesCluster of differentiation 3, CD3, CD3epsilon, IMD18, T3E, TCRE
Cell typeT cell, Tc, Th1, Th2, Th17, Treg, Tfh
Gene ID916