Anti-mouse apoA1 mAb (mHDL36), biotin

Anti‑mouse apoA1 mAb (mHDL36), biotin

3750-6-250

Publications: 9

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Content

Monoclonal antibody mHDL36, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Complementary products

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Anti-mouse apoA1 mAb (mHDL93), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse apoA1 in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

mHDL36 is recommended as detection mAb in ELISA in combination with capture mAb mHDL93 (product code 3750-3). The antibody is also suitable for Western blot.

Product details

Product	Anti-mouse apoA1 mAb (mHDL36), biotin
Application	ELISA, Western blot
Analyte	apoA1
Antibody	mHDL36
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native mouse apoA1
Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Specificity	Mouse apoA1
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody mHDL36, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of mouse apoA1 with mAb mHDL36 in Western blot. Mouse serum (0.1 µl and 0.01 µl) in SDS sample buffer were run on SDS-page / Western blot. ApoA1 was detected using 1 µg/ml mHDL36 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 14, 2022

Apolipoprotein dilution

Recommendations for Mabtech's apolipoprotein ELISAs

Product highlights

Highlighted research, Published September 27, 2018

Immunological effects of hyperlipidemia

First citations for Mabtech ELISA mouse apoE and apoA1 investigating immunological effects of hyperlipidemia.

Publications (9)

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Analyte information

apoA1

Analyte description	Apolipoprotein A1 (apoA1) is the main protein component of high-density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into circulation where it acquires lipids and becomes HDL. A high level of apoA1 is considered protective against atherosclerosis and cardiovascular disease. The normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Alternative names	Apolipoprotein A1, apoA1, apolipoprotein-A1, apo A1, apoA-I, Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, apo-AI
Gene ID	11806