Anti-mouse Ig λ chain mAb (JC5-1), biotin

3895-6-250

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PepPool: CEF (CD8), human

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse Ig lambda in antigen-specific ELISA.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

Product	Anti-mouse Ig λ chain mAb (JC5-1), biotin
Application	ELISA
Analyte	Ig
Antibody	JC5-1
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Mouse lambda light chain
Host	Rat
Isotype	IgG2b
Reactivity	Mouse
Specificity	Mouse Ig lambda light chain
Cross-reactivity	No cross-reactivity (less than 0.1%) with Ig from rat, goat, rabbit, cow, sheep, horse, or human
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody JC5-1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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Analyte information

Analyte description	An antibody (also called immunoglobulin) is a glycoprotein expressed by B cells. Each antibody consists of four polypeptide chains, with two identical copies of both the light and heavy chains. Intra- and inter-chain disulfide bonds link the four polypeptide chains together to one antibody. The heavy chain decides the antibody’s isotype and subclass. The light chains of an antibody are identical and can be of two types; kappa (κ) or lambda (λ). The ratio of kappa: lambda in serum immunoglobulins varies significantly among species.
Alternative names	Immunoglobulin, Ig
Cell type	B cell