Anti-mouse Ig λ chain mAb (JC5-1), biotin

Anti‑mouse Ig λ chain mAb (JC5‑1), biotin

3895-6-250

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Content

Monoclonal antibody JC5-1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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PepPool: CEF (CD8), human


Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse Ig lambda in antigen-specific ELISA.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

ProductAnti-mouse Ig λ chain mAb (JC5-1), biotin
ApplicationELISA
AnalyteIg
AntibodyJC5-1
ConjugateBiotin
ClonalityMonoclonal
ImmunogenMouse lambda light chain
HostRat
IsotypeIgG2b
ReactivityMouse
SpecificityMouse Ig lambda light chain
Cross-reactivityNo cross-reactivity (less than 0.1%) with Ig from rat, goat, rabbit, cow, sheep, horse, or human
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody JC5-1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS
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Ig

Analyte description

An antibody (also called immunoglobulin) is a glycoprotein expressed by B cells. Each antibody consists of four polypeptide chains, with two identical copies of both the light and heavy chains. Intra- and inter-chain disulfide bonds link the four polypeptide chains together to one antibody. The heavy chain decides the antibody’s isotype and subclass. The light chains of an antibody are identical and can be of two types; kappa (κ) or lambda (λ). The ratio of kappa: lambda in serum immunoglobulins varies significantly among species. 

Alternative namesImmunoglobulin, Ig
Cell typeB cell