Anti-mouse IgG1 mAb (MG1), biotin

Anti-mouse IgG1 mAb (MG1), biotin

3821-6-250

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Validated for our assays.

Content

Monoclonal antibody MG1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IgG1 mAb (MG1), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG1 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG1 is recommended as detection mAb in ELISpot in combination with capture mAbs MT24/JC5-1 (product code 3876-3) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-mouse IgG1 mAb (MG1), biotin
ApplicationELISpot, ELISA
AnalyteIgG1
AntibodyMG1
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative mouse IgG
HostRat
IsotypeIgG1
ReactivityMouse
Specificity

Mouse IgG1

Cross-reactivityNo cross-reactivity (less than 0.1%) with mouse IgG2a, IgG2b, IgG2c (IgG2a analogue in e.g. C57BL/6 and NOD mice), IgG3, IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, and human.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MG1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG1. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG1 antibody-secreting cells.

 

Application guide: ELISpot

 

Antigen-specific IgG1

Coating:
Antigen
Detection mAb:
MG1, biotin

mouse igg1 1

 

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
Antigen, biotin

mouse igg1 2

Total IgG1

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
MG1, biotin

mouse igg1 3

 

 

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG1

Analyte description

Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with the highest serum concentrations of IgG1, followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG1 binds to Fc receptors with high affinity and may also activate the complement system.

Alternative namesImmunoglobulin G1, IgG1, Igh-4, VH7183
Cell typeB cell
Gene ID16017