Anti-mouse IgG2a mAb (MG2a), biotin

Anti‑mouse IgG2a mAb (MG2a), biotin

3822-6-250

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Content

Monoclonal antibody MG2a, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IgG2a mAb (MG2a), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG2a in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG2a is recommended as detection mAb in ELISpot in combination with capture mAbs MT24/JC5-1 (product code 3876-3) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-mouse IgG2a mAb (MG2a), biotin
ApplicationELISpot, ELISA
AnalyteIgG2a
AntibodyMG2a
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative mouse IgG
HostRat
IsotypeIgG2a
ReactivityMouse
Specificity

Mouse IgG2a

Cross-reactivityNo cross-reactivity (less than 0.1%) with mouse IgG1, IgG2b, IgG3, IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, and human. Low cross-reactivity (2.5%) with IgG2c (IgG2a analogue in e.g., C57BL/6 and NOD mice).
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MG2a, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2a. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2a antibody-secreting cells.

 

Application guide: ELISpot

 

Ag-specific IgG2a

Coating:
Antigen
Detection mAb:
MG2a, biotin

mouse igg2a 1

 

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
Antigen, biotin

mouse igg2a 2

Total IgG2a

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
MG2a, biotin

mouse igg2a 3

 

 

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG2a

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b, and IgG3.  The IgG2a allele is expressed in e.g., Balb/c mice.

Alternative namesImmunoglobulin G2a, IgG2a, 1810060O09Rik, Igh-1, Igh-1a
Cell typeB cell
Gene ID380793