Anti-mouse IgG2c mAb (MTG2c), biotin

Anti‑mouse IgG2c mAb (MTG2c), biotin

3824-6-250

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Content

Monoclonal antibody MTG2c, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IgG2c mAb (MTG2c), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG2c in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG2c is recommended as detection mAb in ELISpot in combination with capture mAbs MT24/JC5-1 (product code 3876-3) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-mouse IgG2c mAb (MTG2c), biotin
Application	ELISpot, ELISA
Analyte	IgG2c
Antibody	MTG2c
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native mouse IgG
Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Specificity	Mouse IgG2c
Cross-reactivity	No cross-reactivity (less than 0.1%) with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, horse, and human.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MTG2c, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Mouse IgG2c mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2c. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2c antibody-secreting cells.

Application guide: ELISpot

Antigen-specific IgG2c

Coating:
Antigen
Detection mAb:
MTG2c, biotin

Coating mAbs:
MT24/JC5-1, unconjugated
Detection:
Antigen

Total IgG2c

Coating mAbs:
MT24/JC5-1, unconjugated
Detection mAb:
MTG2c, biotin

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (1)

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Analyte information

IgG2c

Analyte description	IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.
Alternative names	Immunoglobulin G2c, IgG2c
Cell type	B cell