Anti-mouse IgG2c mAb (MTG2c), unconjugated

Anti‑mouse IgG2c mAb (MTG2c), unconjugated

3824-3-250

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Content

Monoclonal antibody MTG2c, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG2c in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG2c is recommended as capture mAb in ELISpot (detection with biotinylated antigen). The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-mouse IgG2c mAb (MTG2c), unconjugated
ApplicationELISpot, ELISA
AnalyteIgG2c
AntibodyMTG2c
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative mouse IgG
HostRat
IsotypeIgG2a
ReactivityMouse
Specificity

Mouse IgG2c

Cross-reactivityNo cross-reactivity (less than 0.1%) with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, horse, and human.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTG2c, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2c. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2c antibody-secreting cells.

 

Application guide: ELISpot

 

Antigen-specific IgG2c

Coating:
Antigen
Detection mAb:
MTG2c, biotin

mouse igg2c 1

 

Coating mAbs:
MT24/JC5-1, unconjugated
Detection:
Antigen

mouse igg2c 2

Total IgG2c

Coating mAbs:
MT24/JC5-1, unconjugated
Detection mAb:
MTG2c, biotin

mouse igg2c 3

 

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG2c

Analyte description

IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins.  In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.

Alternative namesImmunoglobulin G2c, IgG2c
Cell typeB cell