Anti-mouse IgG3 mAb (MG3), biotin

3826-6-250

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Validated for our assays.

Content

Monoclonal antibody MG3, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IgG3 mAb (MG3), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG3 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG3 is recommended as detection mAb in ELISpot in combination with capture mAbs MT24/JC5-1 (product code 3876-3) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-mouse IgG3 mAb (MG3), biotin
Application	ELISpot, ELISA
Analyte	IgG3
Antibody	MG3
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native mouse IgG3
Host	Rat
Isotype	IgG1
Reactivity	Mouse
Specificity	Mouse IgG3
Cross-reactivity	No cross-reactivity (less than 0.1%) with mouse IgG1, IgG2a, IgG2b, IgG2c (IgG2a analogue in e.g., C57BL/6 and NOD mice), IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, and human.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MG3, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Mouse IgG3 mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG3. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG3 antibody-secreting cells.

Application guide: ELISpot

Antigen-specific IgG3

Coating:
Antigen
Detection mAb:
MG3, biotin

Coating mAbs:
MT24/JC5-1, unconjugated
Detecting:
Antigen-biotin

Total IgG3

Coating mAbs:
MT24/JC5-1, unconjugated
Detecting:
MG3, biotin

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

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Analyte information

IgG3

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG3 binds to Fc receptors with high affinity and is a good complement activator.
Alternative names	Immunoglobulin G3, IgG3
Cell type	B cell
Gene ID	380795