Anti-mouse IgG3 mAb (MG3), biotin

Anti‑mouse IgG3 mAb (MG3), biotin

3826-6-250

Publications: 0
Documents
Questions?
Validated for our assays.

Content

Monoclonal antibody MG3, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 4

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgG3 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MG3 is recommended as detection mAb in ELISpot in combination with capture mAbs MT24/JC5-1 (product code 3876-3) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-mouse IgG3 mAb (MG3), biotin
ApplicationELISpot, ELISA
AnalyteIgG3
AntibodyMG3
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative mouse IgG3
HostRat
IsotypeIgG1
ReactivityMouse
Specificity

Mouse IgG3

Cross-reactivityNo cross-reactivity (less than 0.1%) with mouse IgG1, IgG2a, IgG2b, IgG2c (IgG2a analogue in e.g., C57BL/6 and NOD mice), IgA, IgE, IgM or with IgG from rat, goat, rabbit, cow, and human.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MG3, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG3. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG3 antibody-secreting cells.

 

Application guide: ELISpot
 
 

Antigen-specific IgG3

Coating: 
Antigen
Detection mAb:
MG3, biotin

Ag-specific mouse igG3

 

Coating mAbs:
MT24/JC5-1, unconjugated
Detecting:
Antigen-biotin

mouse igg3 2

Total IgG3

Coating mAbs:
MT24/JC5-1, unconjugated
Detecting:
MG3, biotin

mouse igg3 3

 

 

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
Loading publications...

IgG3

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG3 binds to Fc receptors with high affinity and is a good complement activator.

Alternative namesImmunoglobulin G3, IgG3
Cell typeB cell
Gene ID380795