Anti-porcine IgG mAb (MT421), unconjugated

Anti-porcine IgG mAb (MT421), unconjugated

3151-3-250

Publications: 6
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Validated for our assays.

Content

Monoclonal antibody MT421, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

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Anti-porcine IgG mAb (MT424), biotin


Product specifications

Intended use

This monoclonal antibody enables specific detection of porcine IgG in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT421 is recommended as capture mAb in ELISpot in combination with biotinylated detection mAb MT424 (product code 3151-6). In ELISA, MT421 is recommended as capture mAb in combination with HRP-conjugated detection mAb MT424 (product code 3151-9H).

Product details

ProductAnti-porcine IgG mAb (MT421), unconjugated
ApplicationELISpot, ELISA
AnalyteIgG
AntibodyMT421
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative pig IgG
HostMouse
IsotypeIgG1
ReactivityPig
Specificity

Porcine (pig) IgG

Cross-reactivity

Cross-reacts with IgG from horse (30% cross-reactivity) and goat (5% cross-reactivity). For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT421, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IGHG, IGHG1, IGHG1.02, IGHJ1, IgG1b
Cell typeB cell
Gene ID396781