Anti-porcine IgG mAb (MT424), biotin

Anti‑porcine IgG mAb (MT424), biotin

3151-6-250

Publications: 4
Documents
Questions?
Validated for our assays.

Content

Monoclonal antibody MT424, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 1

Product specifications

Intended use

This monoclonal antibody enables specific detection of porcine IgG in ELISpot.

Serum/Plasma samples

Recommendation

MT424 is recommended as detection mAb in ELISpot in combination with capture mAb MT421 (product code 3151-3).

Product details

ProductAnti-porcine IgG mAb (MT424), biotin
ApplicationELISpot
AnalyteIgG
AntibodyMT424
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative pig IgG
HostMouse
IsotypeIgG2a
ReactivityPig
Specificity

Porcine (pig) IgG

Cross-reactivity

No cross-reactivity (less than 0.1%) with IgG from cow, horse, sheep, goat, human, or rat. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT424, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
Loading publications...

IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IGHG, IGHG1, IGHG1.02, IGHJ1, IgG1b
Cell typeB cell
Gene ID396781
1 / 3