Anti-rabbit IFN-γ mAb (MT318), biotin

3110-6-250

Publications: 14

Documents

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Validated for our assays.

Content

Monoclonal antibody MT318, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Complementary products

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Anti-rabbit IFN-γ mAb (MT327), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of rabbit IFN-γ in immunoassays such as ELISpot, ELISA, and flow cytometry.

Serum/Plasma samples

Recommendation

MT318 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT327 (product code 3110-3). MT318 is also suitable for flow cytometry.

Product details

Product	Anti-rabbit IFN-γ mAb (MT318), biotin
Application	ELISpot, ELISA, Flow cytometry
Analyte	IFN-γ
Antibody	MT318
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant rabbit IFN-γ
Host	Mouse
Isotype	IgG2a
Reactivity	Rabbit
Specificity	Native and recombinant rabbit IFN-γ
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT318, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (14)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	100008602