Anti-rat IgG2a mAb (MT168B1), biotin

3232-6-250

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Anti-rat IgG2a mAb (MT171A1), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of rat IgG2a in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT168B1 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT171A1 (product code 3232-3).

Product details

Product	Anti-rat IgG2a mAb (MT168B1), biotin
Application	ELISpot, ELISA
Analyte	IgG2a
Antibody	MT168B1
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native rat IgG
Host	Mouse
Isotype	IgG1
Reactivity	Rat
Specificity	Rat IgG2a
Cross-reactivity	No cross-reactivity (less than 0.1%) with rat IgG1, IgG2b, IgG2c, IgA, IgM, IgE or with IgG from mouse, cow, rabbit, horse, goat, human, monkey, cat, and dog
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT168B1, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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Tutorials

Tutorial, Published December 28, 2023

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Analyte information

IgG2a

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2a allele is expressed in e.g., Balb/c mice.
Alternative names	Immunoglobulin G2a, IgG2a
Cell type	B cell
Gene ID	367586