Anti-SARS-CoV-2 (N) mAb (MTCC25), biotin

Anti-SARS-CoV-2 (N) mAb (MTCC25), biotin

4401-6-100

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Product specifications

Intended use

This monoclonal antibody enables specific detection of the SARS-CoV-2 nucleocapsid protein in FociSpot immunostaining of virus infected cells. The antibody can also be used in Western blot and ELISA.

Serum/Plasma samples

Applications
FociSpot immunostaining of SARS-CoV-2 nucleocapsid in focus forming assays. Antibody functionality in FociSpot was confirmed using SARS-CoV-2 infected Vero cells.

Functionality in Western blot and ELISA was confirmed using recombinant protein.

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Product details

ProductAnti-SARS-CoV-2 (N) mAb (MTCC25), biotin
ApplicationFociSpot, Western blot, ELISA
AnalyteSARS-CoV-2 (N)
AntibodyMTCC25
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant SARS-CoV-2 nucleocapsid protein

HostMouse
IsotypeIgG1
ReactivityNaN
Specificity

Native and recombinant SARS-CoV-2 nucleocapsid protein

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTCC25, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/ProtocolSDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.
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SARS-CoV-2 (N)

Analyte description

The nucleocapsid (N) protein is a structural protein of the SARS-CoV-2 virus. It is responsible for packaging the positive-sense RNA genome by forming ribonucleoprotein (RNP) structures with the viral RNA. The N protein is mainly expressed in the host cells' cytoplasm and interacts with the M protein during assembly. The nucleocapsid protein is highly immunogenic and antibodies against it are found in people that recovered from COVID-19. The N protein has been suggested to suppress type I interferon responses. 

Alternative namesNucleocapsid protein, N protein (of SARS-CoV-2)