ELISpot Plus: Bovine IL‑2 (ALP)

Bovine Interleukin-2 (IL-2) ELISpot. PBMC from cow incubated with and without Phytohaemagglutinin (PHA).

ELISpot Plus: Bovine IL‑2 (ALP)

3111-4APW-2

Publications: 0
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT11A31)
Detection mAbMT3B3, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

Low stock

Delivery within 3-4 weeks

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Bovine Interleukin-2 (IL-2) ELISpot. PBMC from cow incubated with and without Phytohaemagglutinin (PHA).
PBMC from cow were incubated in the presence or absence of Phytohaemagglutinin (PHA) and the number of cells secreting IL-2 was analyzed by bovine IL-2 ELISpot.
Bovine Interleukin-2 (IL-2) ELISpot. PBMC from cow incubated with and without Phytohaemagglutinin (PHA).
Datasheet/ProtocolSDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
Loading publications...

IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID280822