ELISpot Plus: Bovine IL‑2 (HRP)

ELISpot Plus: Bovine IL‑2 (HRP)

3111-4HPW-2

Publications: 0
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Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT11A31)
Detection mAbMT3B3, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

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Delivery within 3 weeks

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-2 (HRP)
ApplicationELISpot
AnalyteIL-2
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 7, 2022

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Tutorial, Published October 14, 2022

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DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID280822