ELISA Flex: Bovine IL-4 (HRP)

ELISA Flex: Bovine IL‑4 (HRP)

3118-1H-6

Publications: 10
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbbIL4-I
Detection mAbbIL4-II, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant bovine IL-4 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of bovine IL-4 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Bovine IL-4 (HRP)
ApplicationELISA
AnalyteIL-4
ReactivityCow, Sheep
SpecificityThis kit is based on a matched pair of mAbs specific for bovine (cow) IL-4.
Cross-reactivityThe mAbs cross-react with IL-4 from sheep. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range20-2000 pg/ml
Representative standard curve for bovine IL-4 ELISA Flex kit using TMB substrate.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1
Cell typeT cell, Th2, Treg
Gene ID280824, 443327