ELISpot Plus: Bovine IL-4 (ALP)

ELISpot Plus: Bovine IL‑4 (ALP)

3118-4APW-2

Publications: 3
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb bIL4-I)
Detection mAbbIL4-II, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-4 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-4 (ALP)
ApplicationELISpot
AnalyteIL-4
ReactivityCow, Sheep
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-4.

Cross-reactivity

The mAbs cross-react with IL-4 from sheep. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Bovine PBMC were incubated overnight in the absence (200,000 cells/well) or presence (100,000 cells/well) of Phytohaemagglutinin (PHA) and the number of cells secreting IL-4 was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1
Cell typeT cell, Th2, Treg
Gene ID280824, 443327