ELISpot Plus: Bovine IL-8 (ALP)

ELISpot Plus: Bovine IL‑8 (ALP)

3114-4APW-2

Publications: 1
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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT8H6/8F19)
Detection mAb26E5, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-8 (CXCL8) using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-8 (ALP)
ApplicationELISpot
AnalyteIL-8 (CXCL8)
ReactivityCow, Dog
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of bovine (cow) IL-8 (CXCL8).
Cross-reactivityThe mAbs cross-react with IL-8 from dog. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-8 (CXCL8)

Analyte description

Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.

Alternative namesInterleukin-8, IL-8, IL8, CXCL8
Cell typeT cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID280828, 403850