ELISA Flex: Canine IFN‑γ (ALP)

ELISA Flex: Canine IFN‑γ (ALP)

3113-1A-6

Publications: 4
Documents
Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT13
Detection mAbMT166, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant canine IFN-γ ELISA standard
Buffer/SolutionStandard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of canine IFN-γ in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Canine IFN-γ (ALP)
ApplicationELISA
AnalyteIFN-γ
ReactivityDog
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of canine (dog) IFN-γ.

Cross-reactivity

The mAbs react with horse IFN-γ and cross-react with IFN-γ from dog. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range8-800 pg/ml
Representative standard curve for canine IFN-γ ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Highlighted research, Published May 28, 2019

Canine ELISA in drug discovery
Controlling fusion protein stability and pharmacokinetic with Mabtech ELISA Flex: Canine IFN-γ.

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFN-G
Cell typeT cell, Tc, Th1, NK cell
Gene ID403801