ELISpot Plus: Canine IFN-γ (HRP)
ELISpot Plus: Canine IFN‑γ (HRP)
Publications: 3
Documents
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The easiest way to achieve reproducibility in ELISpot.
Components
| Plate | Pre-coated ELISpot plate, white MSIP (mAb MT13) |
| Detection mAb | MT166, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Substrate | ELISpot substrate: TMB for HRP |
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Performance
PBMC from dog were incubated in the presence (10,000 cells/well) or absence (30,000 cells/well) of PMA/ionomycin and the number of cells secreting IFN-γ was analyzed by canine IFN-γ ELISpot.
Documents
Veterinary cross-reactivity guide
Tutorials
Publications (3)
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFN-G |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 403801 |