ELISpot Plus: Cotton rat IFN‑γ (HRP)
ELISpot Plus: Cotton rat IFN‑γ (HRP)
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The easiest way to achieve reproducibility in ELISpot.
Components
| Plate | Pre-coated ELISpot plate, white MSIP (mAb MT413) |
| Detection mAb | MT417, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Substrate | ELISpot substrate: TMB for HRP |
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Performance
Cotton rat spleen cells were incubated overnight in the absence or presence of PMA + ionomycin and the number of cells secreting IFN-γ was analyzed by ELISpot.
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg |
| Cell type | T cell, Tc, Th1, NK cell |