ELISA Flex: Equine IL‑10 (HRP)

3126-1H-6

Publications: 0

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT3515
Detection mAb	MT32AD, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Recombinant human IL-10 ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 1

ELISA substrate: TMB for HRP

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of equine IL-10 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Equine IL-10 (HRP)
Application	ELISA
Analyte	IL-10
Reactivity	Horse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of equine (horse) IL-10.
Cross-reactivity	The mAbs react with monkey IL-10 and cross-react with IL-10 from human and horse. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

2-250 pg/ml

Representative standard curve for equine IL-10 ELISA Flex kit using TMB substrate.

Documents

Datasheet/Protocol SDS

Veterinary cross-reactivity guide

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Featured product, Published April 28, 2022

Expanding our horse portfolio with IL-10 kits

New IL-10 antibodies for horse research out now.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Publications (?)

Loading publications...

Analyte information

IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10
Cell type	T cell, Treg, Monocyte/MΦ, mDC