ELISA Flex: Human IL-1α (HRP)

ELISA Flex: Human IL‑1α (HRP)

3414-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT641/642
Detection mAbMT513, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-1α ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-1α (pro-form and active form) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IL-1α (HRP)
ApplicationELISA
AnalyteIL-1α
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-1α (pro-form and active form).

Cross-reactivity

The assay shows no cross-reactivity with recombinant human IL-1β or IL-18.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range2-200 pg/ml
Representative standard curve for human IL-1α ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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IL-1α

Analyte description

Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation. 

Alternative namesInterleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
Cell typeMonocyte/MΦ, mDC
Gene ID3552