ELISA Flex: Human IL-22 (HRP)

ELISA Flex: Human IL-22 (HRP)

3475-1H-6

Publications: 3
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT12A3
Detection mAbMT7B27, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-22 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-22 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human IL-22 (HRP)
ApplicationELISA
AnalyteIL-22
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-22.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range4-400 pg/ml
Representative standard curve for human IL-22 ELISA Flex kit using TMB substrate.

Tutorial, Published July 31, 2023

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IL-22

Analyte description

The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.

Alternative namesInterleukin-22, IL-22, IL22, IL-21, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18
Cell typeT cell, Th1, Th17, Tfh
Gene ID50616