ELISA Flex: Human Latent TGF-β1 (HRP)

ELISA Flex: Human Latent TGF‑β1 (HRP)

3550-1H-6

Publications: 27
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT593
Detection mAbMT517, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human LAP ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human latent Transforming Growth Factor-β1 (TGF-β1) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody. For quantification of latent TGF-β1 in blood, the use of plasma is recommended since serum contains high levels of latent TGF-β1 released from platelets during sample preparation. Plasma can be obtained using EDTA, citrate or heparin as anti-coagulants. To minimize the platelet content in the sample, an additional centrifugation of plasma at 10,000 x g for 10 minutes is recommended.

Quantification of latent TGF-β1 - no need for sample pre-treatment
TGF-β1 is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). The monoclonal antibodies MT593 and MT517-biotin bind to the LAP entity of human latent TGF-β1. This enables direct quantification of latent TGF-β1 in ELISA with no need of sample pre-treatment.

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Product details

ProductELISA Flex: Human Latent TGF-β1 (HRP)
ApplicationELISA
AnalyteTGF-β1 (Latent TGF-β1)
ReactivityHuman, Non-human primates
Specificity

This kit is based on a matched pair of mAbs specific for the Latency Associated Protein (LAP) of human latent TGF-β1.

Cross-reactivity

The ELISA does not detect human latent TGF-β2 or -β3 or bovine latent TGF-β1. The mAbs cross-react with latent TGF-β1 from non-human primates (NHP). For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range0.5-50 pM
Representative standard curve for human Latent TGF-β1 ELISA Flex kit using TMB substrate.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

 Rhesus MacaqueCynomolgus MacaquePig-tailed MacaqueBaboon*Sooty MangabeyAfrican Green MonkeyNight Monkey*Common MarmosetSquirrel Monkey
ASSAYOld worldNew world
ApoA1      
ApoB       
ApoE (NHP)       
ApoE       
ApoH       
CCL2 (MCP-1)       
CCL4 (MIP-1β) ( ) ( )( )( )( )
CCL22 (MDC) ( ) ( )( )( )( )
CD25       
GM-CSF( )( )   
Granzyme A        
Granzyme B (NHP)       
Granzyme B     
IFN-α2        
IFN-α pan   ( )   
IFN-γ (NHP)
IFN-γ( )
IgA (NHP)       
IgA       
IgG       
IgM       
INS     
IL-1α        
IL-1β        
IL-2 (NHP)
IL-3       
IL-4( )( )( )( )
IL-5( )( )( ) 
IL-6( )( ) ( ) 
IL-8 (NHP)        
IL-8 (ELISA)        
IL-8 (ELISpot)        
IL-10 (NHP)      
IL-10       
IL-12/-23 (p40)      
IL-12 (p70)        
IL-13 (NHP)( )( )( )  
IL-13        
IL-17A (NHP)( )( )   
IL-17A     
IL-17F        
IL-17A/F        
IL-21      
IL-22        
IL-23     
IL-27        
IL-31        
IP-10       
Perforin( )   
TGF-β1 (latent)       
TNF-α (NHP)( )  ( )( )( )( )
          
SINGLE mAbs         
Cell stimulation         
CD3, mAb CD3-1       
CD3, mAb CD3-2       
CD28, mAb CD28-A       
Flow cytometry          
IFN-γ mAb 1-D1K( )( )( )( )( )( )
IL-2 mAb MT8G10( )( )( )( ) ( )( )( )
IL-4 mAb IL4-3( )       
IL-17A mAb MT504        
Perforin mAb Pf-344     
TNF-α mAb MT15B15       
Ig reagents         
IgG1 mAb MTG1218       
IgG1 mAb MT1939       
IgG2 mAb H6200       
IgG2 mAb MTG211E       
IgG3 mAb MTG34       
IgG4 mAb MTG42       
Western blot         
IFN-γ mAb MT111W( )( )( )( )( )( )( )( )( )
TNF-α mAb MT15B15( )( )( )  ( )( )( )( )

Symbol keys:


Cross-reactivity

Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

  • good
  • poor
  • no

Indications of Cross-reactivity

Based on reports from others or by analysis using recombinant proteins.

  • ( ) good
  • ( ) poor
  • ( ) no

Monkey kits

Specifically developed monkey kits are marked with (NHP).

Blank boxes

Represent cross-reactivities not evaluated.

* Comprises several species. Cross-reactivity may have to be verified on a species basis.

Tutorial, Published July 31, 2023

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TGF-β1 (Latent TGF-β1)

Analyte description

Transforming growth factor β1 (TGF-β1) is secreted by many cells and controls functions such as cell growth, cell differentiation, proliferation, and apoptosis. The cytokine TGF-β1 is an important regulator of immune functions. Specifically for T cells, TGF-β1 promotes the differentiation of regulatory T cells, preventing IL-1- and IL-2-dependent proliferation of activated T cells, the activation of helper T cells, and cytotoxic T cells. TGF-β1 is secreted in a latent, biologically inactive form, non-covalently linked with a latency associated protein (LAP). 

Alternative namesTransforming growth factor β1, TGF-β1, TGF beta 1, latency associated peptide, LAP, CED, DPD1, IBDIMDE, TGF-beta1, TGFB, TGFbeta
Cell typeT cell, Th1, Th17, Treg, Tfh, Monocyte/MΦ
Gene ID7040
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