ELISA PathRF: Human IFN-γ
ELISA PathRF: Human IFN‑γ
Components
| Plate | Pre-coated ELISA strip plate (mAb 1-D1K) |
| Detection mAb | MTR-10, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Recombinant human IFN-γ ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Buffers/Solutions | RF-block diluent |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution | |
| Also provided | Adhesive plate covers |
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Performance
| Standard range | 1-1000 pg/ml |
| Sensitivity | 0.6 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 4 standard deviations to the mean OD of background wells. |
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 3458 |