ELISA PathRF: Human IFN‑γ

ELISA PathRF: Human IFN‑γ

3420-1HP-DP-1

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Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

PlatePre-coated ELISA strip plate (mAb 1-D1K)
Detection mAbMTR-10, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate
StandardRecombinant human IFN-γ ELISA standard
SubstrateTMB substrate for ELISA
Buffers/SolutionsRF-block diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human IFN-γ in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

ProductELISA PathRF: Human IFN-γ
ApplicationELISA
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range1-1000 pg/ml
Sensitivity0.6 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 4 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human IFN-γ.
Datasheet/Protocol
SDS

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Featured product, Published March 1, 2022

How to mitigate interference caused by rheumatoid factor
Analyzing samples from patients with ELISA? Watch out for heterophilic antibodies causing false positives. Discover how Mabtech’s kits overcome this issue.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458