ELISA PathRF: Human IL-17A

ELISA PathRF: Human IL‑17A

3520-1HP-DP-1

Publications: 30
Documents
Questions?
Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

PlatePre-coated ELISA strip plate (mAb MT44.6)
Detection mAbMTR-5, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-17A ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsRF-block diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human IL-17A in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

ProductELISA PathRF: Human IL-17A
ApplicationELISA
AnalyteIL-17A
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-17A.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity1 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human IL-17A.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Featured product, Published March 1, 2022

How to mitigate interference by rheumatoid factor
Analyzing samples from patients with ELISA? Watch out for heterophilic antibodies causing false positives. Discover how Mabtech’s kits overcome this issue.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-17A

Analyte description

Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.

Alternative namesInterleukin 17A, IL-17A, IL17A, CTLA-8, CTLA8, IL-17, IL17, ILA17
Cell typeTh17
Gene ID3605