ELISA PathRF: Human IL‑6

3460-1HP-DP-1

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Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

Plate	Pre-coated ELISA strip plate (mAb 13A5)
Detection mAb	MTR-15, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-6 ELISA standard
Substrate	TMB substrate for ELISA
Buffers/Solutions	RF-block diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human IL-6 in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

Product	ELISA PathRF: Human IL-6
Application	ELISA
Analyte	IL-6
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-6.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	10-3160 pg/ml
Sensitivity	2 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 5 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human IL-6.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Product highlights

Featured product, Published March 1, 2022

How to mitigate interference caused by rheumatoid factor

Analyzing samples from patients with ELISA? Watch out for heterophilic antibodies causing false positives. Discover how Mabtech’s kits overcome this issue.

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Analyte information

IL-6

Analyte description	Interleukin 6 (IL-6) is a pleiotropic cytokine produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things, it augments antibody production from activated B cells in vitro.
Alternative names	Interleukin 6, IL-6, IL6, IFB-B502, BSF-2, BCDF, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2
Cell type	B cell, Monocyte/MΦ, mDC
Gene ID	3569